Artículos de revistas
Nanostructured sensors containing immobilized nuclear receptors for thyroid hormone detection
Fecha
2014-05Registro en:
Journal of Biomedical Nanotechnology,Valencia : American Scientific Publishers - ASP,v. 10, n. 5, p. 744-750, May 2014
10.1166/jbn.2014.1774
Autor
Bendo, Luana
Casanova, Monise
Figueira, Ana Carolina M.
Polikarpov, Igor
Zucolotto, Valtencir
Institución
Resumen
Thyroid hormone receptors (TRs) are members of the nuclear receptors (NRs) superfamily, being encoded by two genes: TRα and TRβ. In this paper, the ligand-binding domain (LBD) of the TRβ1 isoform was immobilized on the surface of nanostructured electrodes for TR detection. The platforms containing TRβ1-LBD were applied to the detection of specific ligand agonists, including the natural hormones T3 (triiodothyronine) and T4 (thyroxine), and the synthetic agonists TRIAC (3,5,3'-triiodothyroacetic acid) and GC-1 [3,5-dimethyl-4-(4'-hydroxy-3' -isopropylbenzyl phenoxy) acetic acid]. Detection was performed via impedance spectroscopy. The biosensors were capable of distinguishing between the thyroid hormones T3 and T4, and/or the analogues TRIAC and GC-1 at concentrations as low as 50 nM. The detection and separation of thyroid hormones and analogue ligands by impedance techniques represents an innovative tool in the field of nanomedicine because it allows the design of inexpensive devices for the rapid and real-time detection of distinct ligand/receptor systems.