Artículos de revistas
Expression, purification, crystallization and preliminary X-ray diffraction analysis of Aspergillus terreus endo-β-1,4-glucanase from glycoside hydrolase family 12
Fecha
2014-02Registro en:
Acta Crystallographica F, Chester : International Union of Crystallography - IUCr, v. 70, part. 2, p. 267-270, Feb. 2014
2053-230X
10.1107/S2053230X13034936
Autor
Segato, Fernando
Berto, Gabriela L.
Araújo, Evandro Ares de
Muniz, João Renato Carvalho
Polikarpov, Igor
Institución
Resumen
Endoglucanases are important enzymes that are involved in the modification and degradation of cellulose. Filamentous fungi such as Aspergillus terreus are effective biomass degraders in nature owing to their capacity to produce an enzymatic arsenal of glycoside hydrolases, including endoglucanase from glycoside hydrolase family 12 (GH12). The A. terreus GH12 endoglucanase was cloned and overexpressed in A. nidulans, purified and crystallized. A single crystal was obtained from a solution consisting of 2 M ammonium sulfate, 5%(v/v) 2-propanol. X-ray diffraction data were collected to a resolution of 1.85 Å using synchrotron radiation and a preliminary molecular-replacement solution was obtained in the trigonal space group P3221. The unit-cell parameters were a=b= 103.24, c=48.96 Å.