Artículos de revistas
Calcium potentiates the effect of estradiol on PGF2α production in the bovine endometrium
Fecha
2014-05-05Registro en:
Journal of Animal Science and Biotechnology. 2014 May 05;5(1):25
Autor
Bertan Membrive, Claudia M
da Cunha, Pauline M
Meirelles, Flávio V
Binelli, Mario
Institución
Resumen
Abstract
Background
Estradiol (E2) is required for luteolysis in cows and its injection stimulates prostaglandin F2α (PGF2α) release. The main goal of our study was to investigate the ability of endometrial explants and cells treated with E2 and the calcium ionophore (CI) A23187 to synthesize PGF2α.
Results
Treatment with E2
in vivo resulted in a 48.4% increase of PGF2α production by endometrial explants treated in vitro with A23187. Production of PGF2α was better stimulated with A23187 at concentrations of 10-6 and 10-5 mol/L compared with other concentrations used. The concentration of PGF2α for untreated bovine endometrial cell cultures was 33.1 pg/mL, while for cultures treated with E2, A23187, or a combination of E2 and A23187, the PGF2α concentration was 32.5, 92.4 and 145.6 pg/mL, respectively.
Conclusions
Treatment with A23187 tended to stimulate PGF2α production. In the presence of E2, A23187 significantly stimulated PGF2α synthesis. It appears that A23187 potentiates the effects of E2 with respect to synthesis of endometrial PGF2α in cattle.