Artículos de revistas
Frequency of human platelet antigens in oncohematological patients with thrombocytopenia and the probability of incompatibility to platelet transfusions
Frequência dos antígenos plaquetários humanos (HPA) em pacientes trombocitopênicos e predisposição à incompatibilidade transfusional
Fecha
2012Registro en:
Rev. Bras. Hematol. Hemoter.,v.34,n.3,p.202-205,2012
1516-8484
10.5581/1516-8484.20120050
Autor
Bianchi, Juliana Vieira dos Santos
Azevedo, Maria Regina Andrade de
Jens, Eduardo
Nukui, Youko
Chamone, Dalton Alencar Ficher
Institución
Resumen
OBJECTIVE: The objective of this study was to evaluate the frequencies of human platelet antigens in oncohematological patients with thrombocytopenia and to analyze the probability of their incompatibility with platelet transfusions. METHODS: Platelet antigen genotyping was performed by sequence-specific primer polymerase chain reaction (SSP-PCR) for the HPA-1a, HPA-1b, HPA-2a, HPA-2b, HPA-3a, HPA-3b, HPA-4a, HPA-4b, HPA-5a, HPA-5b; HPA-15a, HPA-15b alleles in 150 patients of the Hematology Service of the Hospital das Clínicas (FMUSP). RESULTS: The allele frequencies found were: HPA-1a: 0.837; HPA-1b: 0.163; HPA-2a: 0.830; HPA-2b: 0.170; HPA-3a: 0.700; HPA-3b: 0.300; HPA-4a: 1; HPA-4b: 0; HPA-5a: 0.887; HPA-5b: 0.113; HPA-15a: 0.457 and HPA-15b: 0.543. CONCLUSIONS: Data from the present study showed that the A allele is more common in the population than the B allele, except for HPA-15. This suggests that patients homozygous for the B allele are more predisposed to present alloimmunization and refractoriness to platelet transfusions by immune causes. Platelet genotyping could be of great value in the diagnosis of alloimmune thrombocytopenia and to provide compatible platelet concentrates for these patients.
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Clot lysis time in platelet-rich plasma: method assessment, comparison with assays in platelet-free and platelet-poor plasmas, and response to tranexamic acid
Panes, Olga; Padilla, Oslando; Matus, Valeria; Saez, Claudia G.; Berkovits, Alejandro; Pereira, Jaime; Mezzano, Diego -
Clot lysis time in platelet-rich plasma: method assessment, comparison with assays in platelet-free and platelet-poor plasmas, and response to tranexamic acid.
Panes, Olga; Padilla, Oslando; Matus, Valeria; Saez, Claudia G.; Berkovits, Alejandro; Pereira, Jaime; Mezzano, Diego