Artículos de revistas
Evaluation of GBV-C / HVG viremia in HIV-infected women
Avaliação da viremia por GBV-C/HGV em mulheres infectadas pelo HIV
Fecha
2012Registro en:
Rev. Inst. Med. trop. S. Paulo,v.54,n.1,p.31-35,2012
0036-4665
10.1590/S0036-46652012000100006
Autor
Silva, Synara Araújo
Rodrigues, Célia Lima
Campos, Aléia Faustino
Levi, José Eduardo
Institución
Resumen
The present study aimed at standardizing a real-time quantitative polymerase chain reaction assay to evaluate the presence of GBV-C/HGV RNA. A "TaqMan" assay using primers and probe derived from the 5¢ NCR region was developed and validated. Two hundred and fifty-three plasma samples from HIV-infected women were tested for GBV-C viremia and antibody against the envelope protein 2. GBV-C RNA was detected in 22.5% of the patients whereas the antibody was identified in 25.3% of the cohort. Detection of viral RNA and of antibodies was mutually exclusive. Viral loads showed a mean of 1,777 arbitrary units / mL, being 1.1 and 13,625 arbitrary units / mL respectively the lowest and highest values measured. We conclude that the real-time quantitative polymerase chain reaction method developed is appropriate for the investigation of GBV-C RNA since it was shown to be highly specific and sensitive, as well as requiring few steps, preventing contamination and providing additional information as to the relative viremia of carriers, a parameter that must be included in studies evaluating the co-factors influencing the clinical outcome of HIV/AIDS.