Artículos de revistas
A Validated Chromatographic Method for the Determination of Flavonoids in Copaifera langsdorffii by HPLC
Fecha
2012Registro en:
NATURAL PRODUCT COMMUNICATIONS, WESTERVILLE, v. 7, n. 1, pp. 25-28, JAN, 2012
1934-578X
Autor
Sousa, João Paulo Barreto de
Brancalion, Ana Paula S.
Groppo Junior, Milton
Bastos, Jairo Kenupp
Institución
Resumen
Hydroethanolic extracts of C. langsdorffii leaves have therapeutic potential. This work reports a validated chromatographic method for the quantification of polar compounds in the hydroethanolic extract of C. langsdorffii leaves. A reliable HPLC method was developed using two monolithic columns linked in series (100 x 4.6 mm - C-18), with nonlinear gradient elution, and UV detection set at 257 nm. A procedure for the extraction of flavonols was also developed, which involved the use of 70% aqueous ethanol and the addition of benzophenone as the internal standard. The developed method led to a good detection response as the values for linearity were between 10.3 and 1000 mu g/mL, and those for recovery between 84.2 and 111.1%. The detection limit ranged from 0.02 to 1.70 mu g/mL and the quantitation limit from 0.07 to 5.1 mu g/mL, with a maximum RSD of 5.24%. Five compounds, rutin, quercetin-3-O-alpha-L-rhamnopyranoside, kaempferol-3-O-alpha-L-rhamnopyranoside, quercetin and kaempferol, were quantified. This method could, therefore, be used for the quality control of hydroethanolic extracts of Copaifera leaves and their cosmetic and pharmaceutical products.