Artículos de revistas
Analysis of the mRNA expression of the S100 beta protein in adipocytes of patients with diabetes mellitus, type 2
Fecha
2012Registro en:
ARQUIVOS BRASILEIROS DE ENDOCRINOLOGIA E METABOLOGIA, RIO DE JANEIRO, RJ, v. 56, n. 7, pp. 435-440, OCT, 2012
0004-2730
10.1590/S0004-27302012000700005
Autor
Hamasaki, Mike Yoshio
Hirata, Mario Hiroyuki
Hirata, Rosario Dominguez Crespo
Himelfarb, Silvia Tchernin
Campos, Leila Maria Guissoni
Nogueira, Maria Ines
Institución
Resumen
Objective: This study aims to explore the possible relationship between the expression level of S100 beta protein mRNA with diabetes mellitus type 2 in adipocytes from patients with this disease in comparison with normoglycemic individuals. Materials and methods: Samples of adipose tissue of eight patients from the coronary section of the Institute Dante Pazzanese of Cardiology (IDPC), four in Group Diabetes and four of Normoglycemic group, were evaluated by RT-PCR real time. Results: An increase around 15 times values, between the threshold cycle (Delta Ct), of mRNA expression of S100 beta protein in adipocytes of the diabetes group was observed in comparison to the control group (p = 0.015). Conclusion: Our results indicate, for the first time, that there is coexistence of increased expression of the S100 beta and the type 2 diabetes mellitus gene. Arq Bras Endocrinol Metab. 2012;56(7):435-40