Artículos de revistas
A dry extract of Phyllanthus niruri protects normal cells and induces apoptosis in human liver carcinoma cells
Fecha
2012Registro en:
EXPERIMENTAL BIOLOGY AND MEDICINE, LONDON, v. 237, n. 11, supl. 1, Part 2, pp. 1281-1288, NOV, 2012
1535-3702
10.1258/ebm.2012.012130
Autor
Araujo Junior, Raimundo Fernandes de
Souza, Tatiane Pereira de
Pires, Júlia Glória Lucatelli
Soares, Luiz Alberto Lira
Araujo, Aurigena Antunes de
Petrovick, Pedro Ros
Macedo, Helainy Daline Oliveira
Oliveira, Ana Luiza Cabral de Sa Leitao
Guerra, Gerlane Coelho Bernardo
Institución
Resumen
The ability to induce apoptosis is an important marker for cytotoxic antitumor agents. Some natural compounds have been shown to modulate apoptosis pathways that are frequently blocked in human cancers, and therefore, these compounds provide novel opportunities for cancer drug development. Phyllanthus, a plant genus of the family Euphorbiaceae, exhibits multiple pharmacological actions. Of these, Phyllanthus niruri extracts exhibit significant antitumor activity, which is consistent with the traditional medicinal use of this plant. To examine the apoptotic effects of a spray-dried extract of P. niruri (SDEPN), human hepatocellular carcinoma cells (HepG2, Huh-7), colorectal carcinoma cells (Ht29) and keratinocytes (HaCaT) were exposed to the extract for 4, 8 and 24 h. Flow cytometry and caspase-3 immunostaining were used to detect apoptosis, while analysis of variance was applied to identify significant differences between groups (P < 0.05). At all timepoints, the SDEPN induced significantly different cytotoxic effects for HepG2 and Huh-7 cells compared with control cells (P < 0.001). In contrast, the SDEPN had a protective effect on HaCaT cells compared with control cells at all timepoints (P < 0.001). In caspase-3 assays, activation was detected after cell death was induced in Huh-7 and HepG2 cancer cells by the SDEPN. In combination, these results indicate that the SDEPN is selectively toxic towards cancer cell lines, yet is protective towards normal cells.