Artículos de revistas
Intracellular Reactive Oxygen Species Production by Polymorphonuclear Leukocytes in Bovine Leukemia Virus-Infected Dairy Cows
Fecha
2012Registro en:
JOURNAL OF VETERINARY MEDICAL SCIENCE, TOKYO, v. 74, n. 2, pp. 221-225, FEB, 2012
0916-7250
10.1292/jvms.11-0246
Autor
Souza, Fernando Nogueira de
Blagitz, Maiara Garcia
Latorre, Andreia Oliveira
Sanchez, Eduardo Milton Ramos
Batista, Camila Freitas
Weigel, Rebeca Alves
Rennó, Francisco Palma
Sucupira, Maria Claudia Araripe
Libera, Alice Maria Melville Paiva Della
Institución
Resumen
The present study assesses the oxidative burst activity from polymorphonuclear leukocytes (PMNLs) from bovine leukemia virus (BLV)-infected cows. Fifteen clinically healthy cows were divided into serologically positive cows without any hematological alteration, serologically positive animals with persistent lymphocytosis (PL) and healthy serologically negative cows. The oxidative burst activity from the PMNLs was evaluated by now cytometry using 2',7'-dichlorofluorescein diacetate as a probe. PMNLs from each cow were incubated with heat-killed Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) to stimulate oxidative burst activity. The results of the present work showed no significant difference in the oxidative burst activity without any stimulus and elicited by S. caucus. Conversely, a decrease in the oxidative burst index induced by E. coli in PMNLs was observed in BLV-infected cows.