Artículos de revistas
EVALUATION OF MICROBIAL GROWTH ON SINGLE-USE VITRECTOMY PROBES REPROCESSED IN HEALTHCARE PRACTICE
Fecha
2012Registro en:
REVISTA DA ESCOLA DE ENFERMAGEM DA USP, CERQUERA CESAR, v. 46, n. 3, supl. 1, Part 2, pp. 597-603, JUN, 2012
0080-6234
10.1590/S0080-62342012000300010
Autor
Gomes Pinto, Flavia Morais
Lopes Araujo, Valeria Garcia
de Souza, Rafael Queiroz
Goveia, Vania Regina
Missali, Carmen Castilho
Luz, Reginaldo
Graziano, Kazuko Uchikawa
Institución
Resumen
The aim of this study was to evaluate the microbial growth on single-use vitrectomy probes reprocessed in healthcare practice. We investigated nine vitrectomy probes that had been reused and reprocessed using different methods. The samples were sectioned, individually, in portions of 3.5 cm, totaling 979 sampling units (extensions, connectors and vitrectomy cutters), which were inoculated in culture medium and incubated at 37 C for 14 days. The results showed microbial growth on 57 (5.8%) sample units, 25 of which had been sterilized using ethylene oxide, 16 by hydrogen peroxide plasma, and 16 by low-temperature steam and formaldehyde. Seventeen microbial species were identified. The most prevalent were: Micrococcus spp., coagulase-negative Staphylococcus, Pseudomonas spp., and Bacillus subtilis. The reuse of single-use vitrectomy probes was shown to be unsafe, therefore this practice is not recommended.