Artículos de revistas
Enzymatic Solid-Phase Reactor Based on Silica Organofunctionalized with p-Phenylenediamine for Electrochemical Detection of Phenolic Compounds
Fecha
2012Registro en:
SENSOR LETTERS, VALENCIA, v. 10, n. 41367, supl. 1, Part 3, pp. 1031-1038, MAR-APR, 2012
1546-198X
10.1166/sl.2012.2345
Autor
Moraes, Fernando C.
Cesarino, Ivana
Golinelli, Diego L. C.
Machado, Sergio Antonio Spinola
Institución
Resumen
A new biomaterial, based on silica organofunctionalized with p-phenylenediamine (p-PDA) and the enzyme peroxidase, was used in the development of an enzymatic solid-phase reactor. The analytical techniques used in the characterization showed that the organic ligand was incorporated into the silica matrix. Thus, the silica modified with p-PDA allowed the incorporation of peroxidase by the electrostatic interaction between the carboxylic groups present in the enzyme molecules and the amino groups attached to the silica. The enzymatic solid-phase reactor was used for chemical oxidation of phenols in 1, 4-benzoquinone that was then detected by chronoamperometry. The system allowed the analysis of hydroquinone with a detection limit of 83.6 nmol L-1. Thus, the new material has potential in the determination of phenolic compounds river water samples.