Artículos de revistas
Translocation capture sequencing: A method for high throughput mapping of chromosomal rearrangements
Fecha
2012Registro en:
JOURNAL OF IMMUNOLOGICAL METHODS, AMSTERDAM, v. 375, n. 41306, supl. 1, Part 3, pp. 176-181, 11324, 2012
0022-1759
10.1016/j.jim.2011.10.007
Autor
Oliveira, Thiago Y.
Resch, Wolfgang
Jankovic, Mila
Casellas, Rafael
Nussenzweig, Michel C.
Klein, Isaac A.
Institución
Resumen
Chromosomal translocations require formation and joining of DNA double strand breaks (DSBs). These events disrupt the integrity of the genome and are involved in producing leukemias, lymphomas and sarcomas. Translocations are frequent, clonal and recurrent in mature B cell lymphomas, which bear a particularly high DNA damage burden by virtue of activation-induced cytidine deaminase (AID) expression. Despite the ubiquity of genomic rearrangements, the forces that underlie their genesis are not well understood. Here, we provide a detailed description of a new method for studying these events, translocation capture sequencing (TC-Seq). TC-Seq provides the means to document chromosomal rearrangements genome-wide in primary cells, and to discover recombination hotspots. Demonstrating its effectiveness, we successfully estimate the frequency of c-myc/IgH translocations in primary B cells, and identify hotspots of AID-mediated recombination. Furthermore. TC-Seq can be adapted to generate genome-wide rearrangement maps in any cell type and under any condition. (C) 2011 Elsevier B.V. All rights reserved.