Artículos de revistas
Immunophenotyping and extracellular matrix remodeling in pulmonary and extrapulmonary sarcoidosis
Fecha
2012Registro en:
JORNAL BRASILEIRO DE PNEUMOLOGIA, BRASILIA DF, v. 38, n. 3, supl. 1, Part 2, pp. 321-330, MAY-JUN, 2012
1806-3713
10.1590/S1806-37132012000300007
Autor
Ramos Quintino da Silva, Pedro Henrique
Parra, Edwin Roger
Zocolaro, William Sanches
Narde, Ivy
Rodrigues, Fabiola
Kairalla, Ronaldo Adib
Ribeiro Carvalho, Carlos Roberto
Capelozzi, Vera Luiza
Institución
Resumen
Objective: To investigate the significance of cellular immune markers, as well as that of collagen and elastic components of the extracellular matrix, within granulomatous structures in biopsies of patients with pulmonary or extrapulmonary sarcoidosis. Methods: We carried out qualitative and quantitative evaluations of inflammatory cells, collagen fibers, and elastic fibers in granulomatous structures in surgical biopsies of 40 patients with pulmonary and extrapulmonary sarcoidosis using histomorphometry, immunohistochemistry, picrosirius red staining, and Weigert's resorcin-fuchsin staining. Results: The extrapulmonary tissue biopsies presented significantly higher densities of lymphocytes, macrophages, and neutrophils than did the lung tissue biopsies. Pulmonary granulomas showed a significantly higher number of collagen fibers and a lower density of elastic fibers than did extrapulmonary granulomas. The amount of macrophages in the lung samples correlated with FVC (p < 0.05), whereas the amount of CD3+, CD4+, and CD8+ lymphocytes correlated with the FEV1/FVC ratio and VC. There were inverse correlations between TLC and the CD1a+ cell count (p < 0.05), as well as between DLCO and collagen/elastic fiber density (r = -0.90; p = 0.04). Conclusions: Immunophenotyping and remodeling both showed differences between pulmonary and extrapulmonary sarcoidosis in terms of the characteristics of the biopsy samples. These differences correlated with the clinical and spirometric data obtained for the patients, suggesting that two different pathways are involved in the mechanism of antigen clearance, which was more effective in the lungs and lymph nodes.