Artículos de revistas
QUANTIFICATION OF TRYPTOPHAN IN PLASMA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Fecha
2013-08-02Registro en:
QUIMICA NOVA, SAO PAULO, v. 35, n. 3, supl. 18, Part 1-2, pp. 623-626, JUN, 2012
0100-4042
10.1590/S0100-40422012000300032
Autor
Pinhati, Renata Romanholi
Polonini, Hudson Caetano
Fernandes Brandao, Marcos Antonio
Barbosa Raposo, Nadia Rezende
Corchs, Felipe
Gattaz, Wagner Farid
Institución
Resumen
A simple, rapid and selective method using high-performance liquid chromatography with ultraviolet detection (267 nm) was applied for the determination of tryptophan in plasma. Separation was carried out on a C18 column (150 x 4.6 mm internal diameter) in 6 min. The mobile phase consisted of 5 mM the sodium acetate and acetonitrile (92:8, v/v). The method was shown to be precise and accurate, and good recovery of analyte was achieved, characterizing the method as efficient and reliable for use in laboratory analysis.