Artículos de revistas
Bone marrow cells of swine: collection and separation
Fecha
2012Registro en:
Microscopy Research and Technique, Hoboken, v. 75, n. 7, pp. 917-920, jul, 2012
1059-910X
10.1002/jemt.22013
Autor
Branco, Erika
Cabral, Rosa
Gomes, Bruno Duarte
Kfoury Junior, José Roberto
Miglino, Maria Angelica
Institución
Resumen
Bone marrow is a source of stem cells for greater and easier access, which is widely studied as a provider of hematopoietic and mesenchymal cells for various purposes, mainly therapeutic by the advances in research involving cell therapy. The swine is an animal species commonly used in the pursuit of development of experimental models. Thus, this study aimed to standardize protocol for collection and separation of bone marrow in swines, since this species is widely used as experimental models for various diseases. Twelve animals were used, which underwent bone marrow puncture with access from the iliac crest and cell separation by density gradient followed by a viability test with an average of 98% of viable cells. Given our results, we can ensure the swine as an excellent model for obtaining and isolation of mononuclear cells from bone marrow, stimulating several studies addressing the field of cell therapy. Microsc. Res. Tech., 2012. (C) 2012 Wiley Periodicals, Inc.