Artículos de revistas
Bioreduction of alpha-chloroacetophenone by whole cells of marine fungi
Fecha
2009Registro en:
BIOTECHNOLOGY LETTERS, v.31, n.10, p.1559-1563, 2009
0141-5492
10.1007/s10529-009-0037-y
Autor
ROCHA, Lenilson C.
FERREIRA, Hercules V.
PIMENTA, Eli F.
BERLINCK, Roberto G. S.
SELEGHIM, Mirna H. R.
JAVAROTI, Darci C. D.
SETTE, Lara D.
BONUGLI, Rafaella C.
PORTO, Andre L. M.
Institución
Resumen
The asymmetric reduction of 2-chloro-1-phenylethanone (1) by seven strains of marine fungi was evaluated and afforded (S)-(-)-2-chloro-1-phenylethanol with, in the best case, an enantiomeric excess of 50% and an isolated yield of 60%. The ability of marine fungi to catalyse the reduction was directly dependent on growth in artificial sea water-based medium containing a high concentration of Cl(-) (1.2 M). When fungi were grown in the absence of artificial sea water, no reduction of 1 by whole cells was observed. The biocatalytic reduction of 1 was more efficient at neutral rather than acidic pH values and in the absence of glucose as co-substrate.