Artículos de revistas
Functional Expression of Chemoreceptors with the Help of a Guanine Nucleotide Exchange Factor
Fecha
2009Registro en:
Annals of the New York Academy of Sciences, v.1170, p.150-152, 2009
978-1-57331-738-2
0077-8923
10.1111/i.1749-6632.2009.03874.x
Autor
MALNIC, Bettina
GONZALEZ-KRISTELLER, Daniela C.
Institución
Resumen
Odorant receptors and other chemoreceptors are usually poorly expressed in the plasma membrane of heterologous cells. A key point of regulation in G protein-mediated signaling is the interconversion between the active GTP-bound and inactive GDP-bound states of the G alpha subunit, which regulatory proteins, such as guanine nucleotide exchange factors (GEFs), can control. GEFs stimulate formation of the GTP-bound state of G alpha and therefore are considered to work as positive regulators of G protein-coupled receptor signaling. Ric-8B, a GEF that is specifically expressed in olfactory sensory neurons, promotes functional expression of odorant receptors in HEK293T cells because it amplifies the initially low receptor signaling through G alpha olf. This same strategy could be used to functionally express other types of chemoreceptors.