Artículos de revistas
Biolabeling with nanoparticles based on Y(2)O(3):Nd(3+) and luminescence detection in the near-infrared
Fecha
2011Registro en:
JOURNAL OF LUMINESCENCE, v.131, n.4, p.727-731, 2011
0022-2313
10.1016/j.jlumin.2010.11.026
Autor
KODAIRA, Claudia A.
LOURENCO, Ana Valeria S.
FELINTO, Maria Claudia F. C.
SANCHEZ, Eduardo M. R.
RIOS, Francisco J. O.
NUNES, Luiz Antonio O.
GIDLUND, Magnus
MALTA, Oscar L.
BRITO, Hermi F.
Institución
Resumen
Neodymium based fluorescence presents several advantages in comparison to conventional rare earth or enzyme-substrate based fluorescence emitting sources (e.g.Tb, HRP). Based on this fact we have herein explored a Nd-based fluoroimmunoassay. We efficiently detected the presence of an oxidized low-density lipoprotein (oxLDL) in human plasma a well-known marker for cardiovascular diseases, which causes around 30% of deaths worldwide. Conventional fluoroimmunoassay uses time-resolved luminescence techniques, with detection in the visible range, to eliminate the fluorescence background from the biological specimens. By using an immunoassay based on functionalized Y(2)O(3):Nd(3+) nanoparticles, where the excitation and emission processes in the Nd(3+) ion occur in the near-infrared (NIR) region, we have succeeded in eliminating the interferences from the biological fluorescence background, avoiding the use of time-resolved techniques. This yields higher emission intensity from the Nd(3+)-nanolabels and efficient detection of anti-oxidized low-density lipoproteins (anti-oxLDL) by Y(2)O(3):Nd(3+)-antibody-antigen conjugation, leading to a novel biolabeling method. (C) 2010 Elsevier B.V. All rights reserved.