Artículos de revistas
Geranylation of benzoic acid derivatives by enzymatic extracts from Piper crassinervium (Piperaceae)
Fecha
2010Registro en:
BIORESOURCE TECHNOLOGY, v.101, n.12, p.4251-4260, 2010
0960-8524
10.1016/j.biortech.2010.01.041
Autor
LOPEZ, Silvia Noeli
LOPES, Adriana Aparecida
BATISTA JR., Joao Marcos
FLAUSINO JR., Otavio
BOLZANI, Vanderlan da Silva
KATO, Massuo Jorge
FURLAN, Maysa
Institución
Resumen
The ability to carry out geranylations on aromatic substrates using enzymatic extracts from the leaves of Piper crassinervium (Piperaceae) was evaluated. A literature analysis pointed out its importance as a source of prenylated bioactive molecules. The screening performed on aromatic acceptors (benzoic acids, phenols and phenylpropanoids) including geranyl diphosphate as prenyl donor, showed the biotransformation of the 3,4-dihydroxybenzoic acid by the crude extract, and the p-hydroxybenzoic acid by both the microsomal fraction and the crude extract, after treating leaves with glucose. The analysis of the products allowed the identification of C- and O-geranylated derivatives, and the protease (subtilisin and pepsin) inhibition performed on the O-geranylated compounds showed weak inhibition. Electrophoretic profiles indicated the presence of bands/spots among 56-58 kDa and pI 6-7, which are compatible with prenyltransferases. These findings show that P. crassinervium could be considered as a source of extracts with geranyltransferase activity to perform biotransformations on aromatic substrates. (C) 2010 Elsevier Ltd. All rights reserved.