Artículos de revistas
Establishment of the comet assay in the freshwater snail Biomphalaria glabrata (Say, 1818)
Fecha
2008Registro en:
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v.654, n.1, p.58-63, 2008
1383-5718
10.1016/j.mrgentox.2008.05.007
Autor
GRAZEFFE, Vanessa Siqueira
TALLARICO, Lenita de Freitas
PINHEIRO, Alessandro de Sa
KAWANO, Toshie
SUZUKI, Miriam Fussae
OKAZAKI, Kayo
PEREIRA, Carlos Alberto de Braganca
NAKANO, Eliana
Institución
Resumen
The single cell gel eletrophoresis or the comet assay was established in the freshwater snail Biomphalaria glabrata. For detecting DNA damage in circulating hemocytes, adult snails were irradiated with single doses of 2.5. 5, 10 and 20 Gy of Co-60 gamma radiation. Genotoxic effect of ionizing radiation was detected at all doses as a dose-related increase in DNA migration. Comet assay in B. glabrata demonstrated to be a simple, fast and reliable tool in the evaluation of genotoxic effects of environmental mutagens. (c) 2008 Elsevier B.V. All rights reserved.