Artículos de revistas
Use of PCR for detecting Aspergillus flavus in maize treated by gamma radiation process
Fecha
2008Registro en:
FOOD BIOTECHNOLOGY, v.22, n.1/Fev, p.150-159, 2008
0890-5436
10.1080/08905430802043164
Autor
AQUINO, Simone
GREINER, Ralf
KONIETZNY, Ursula
HASSEGAWA, Regina H.
REIS, Tatiana Alves dos
CORREA, Benedito
VILLAVICENCIO, Anna Lucia C. H.
Institución
Resumen
The aim of this study was to verify the effects of gamma radiation process on the fungal DNA and the application of PCR in the detection of Aspergillus flavus in irradiated maize grains. The samples were inoculated with a toxigenic strain and incubated under controlled conditions of relative humidity, water activity, and temperature for 15 days. After incubation, the samples were treated with gamma radiation with doses of 5 and 10 kGy and individually analyzed. The use of PCR technique showed the presence of DNA bands of Aspergillus flavus in all irradiated samples that showed no fungal growth in agar medium.