Artículos de revistas
Genotoxic effects of X-rays on keratinized mucosa cells during panoramic dental radiography
Fecha
2008Registro en:
DENTOMAXILLOFACIAL RADIOLOGY, v.37, n.7, p.398-403, 2008
0250-832X
10.1259/dmfr/56848097
Autor
CERQUEIRA, E. M. M.
MEIRELES, J. R. C.
LOPES, M. A.
JUNQUEIRA, V. C.
GOMES-FILHO, Is
TRINDADE, S.
MACHADO-SANTELLI, G. M.
Institución
Resumen
Objectives: The aim of this study was to evaluate the genotoxic effects of X-rays on epithelial gingival cells during panoramic dental radiography using a differentiated protocol for the micronucleus test. Methods: 40 healthy individuals who underwent this procedure for diagnostic purposes on request from their dentists agreed to participate in this study. All of them answered a questionnaire before the examination. Epithelial gingival cells were obtained from the keratinized mucosa of the upper dental arcade by gentle scraping with a cervical brush immediately before exposure and 10 days later. Cytological preparations were stained according to the Feulgen-Rossenbeck reaction, counterstained with fast green 1% for 1 min and analysed under a light microscope. Micronuclei, nuclear projections (broken eggs) and degenerative nuclear alterations (pyknosis, karyolysis, karyorrhexis and condensed chromatin) were scored. Results: The frequency of micronuclei was significantly higher after exposure (P < 0.05), as were frequencies of nuclear alterations indicate of apoptosis (P < 0.001). Conclusions: These results indicate that X-ray radiation emitted during panoramic dental radiography induces a genotoxic effect on epithelial gingival cells that increases the frequency of chromosomal damage and nuclear alterations indicative of apoptosis.