Artículos de revistas
L-NAME-treatment alters ectonucleotidase activities in kidney membranes of rats
Fecha
2010Registro en:
LIFE SCIENCES, v.87, n.9/Out, p.325-332, 2010
0024-3205
10.1016/j.lfs.2010.07.008
Autor
FURSTENAU, Cristina Ribas
RAMOS, Denise Barbosa
VUADEN, Fernanda Cenci
CASALI, Emerson Andre
MONTEIRO, Priscilla de Souza
TRENTIN, Danielle da Silva
GOSSENHEIMER, Agnes Nogueira
BOGO, Mauricio Reis
BONAN, Carla Denise
BARRETO-CHAVES, Maria Luiza Morais
SARKIS, Joao Jose Freitas
WOFCHUK, Susana Tchernin
Institución
Resumen
Aims: To investigate the effect of N omega-Nitro-L-arginine methyl ester CL-NAME) treatment, known to induce a sustained elevation of blood pressure, on ectonucleotidase activities in kidney membranes of rats. Main methods: L-NAME (30 mg/kg/day) was administered to Wistar rats for 14 days in the drinking water. Enzyme activities were determined colorimetrically and their gene expression patterns were analyzed by semi-quantitative RT-PCR. The metabolism of ATP and the accumulation of adenosine were evaluated by HPLC in kidney membranes from control and hypertensive rats. PKC phosphorylation state was investigated by Western blot. Key findings: We observed an increase in systolic blood pressure from 115 +/- 12 mmHg (control group) to 152 18 mmHg (L-NAME-treated group). Furthermore, the hydrolysis of ATP, ADP, AMP, and p-Nph-5`TMP was also increased (17%, 35%, 27%, 20%, respectively) as was the gene expression of NTPDase2, NTPDase3 and NPP3 in kidneys of hypertensive animals. Phospho-PKC was increased in hypertensive rats. Significance: The general increase in ATP hydrolysis and in ecto-5`-nucleotidase activity suggests a rise in renal adenosine levels and in renal autoregulatory responses in order to protect the kidney against the threat presented by hypertension. (C) 2010 Elsevier Inc. All rights reserved.