Artículos de revistas
Use of the DNA Checkerboard hybridization method for detection and quantitation of Candida species in oral microbiota
Fecha
2009Registro en:
CANADIAN JOURNAL OF MICROBIOLOGY, v.55, n.5, p.622-626, 2009
0008-4166
10.1139/W08-160
Autor
NASCIMENTO, Cassio do
ALBUQUERQUE JUNIOR, Rubens Ferreira de
ISSA, Joao Paulo Mardegan
ITO, Izabel Yoko
SILVA, Claudia Helena Lovato da
PARANHOS, Helena de Freitas Oliveira
SOUZA, Raphael Freitas de
Institución
Resumen
The DNA Checkerboard method enables the simultaneous identification of distinct microorganisms in a large number of samples and employs up to 45 whole genomic DNA probes to gram-negative and gram-positive bacterial species present in subgingival biofilms. Collectively, they account for 55%-60% of the bacteria in subgingival biofilms. In this study, we present the DNA Checkerboard hybridization as an alternative method for the detection and quantitation of Candida species in oral cavities. Our results reveal that DNA Checkerboard is sensitive enough and constitutes a powerful and appropriate method for detecting and quantifying Candida species found in the oral cavity.