Artículos de revistas
Deficiency of IL-12p40 subunit determines severe paracoccidioidomycosis in mice
Fecha
2008Registro en:
MEDICAL MYCOLOGY, v.46, n.7, p.637-646, 2008
1369-3786
10.1080/13693780801982762
Autor
LIVONESI, Marcia Cristina
SOUTO, Janeusa Trindade
CAMPANELLI, Ana Paula
MAFFEI, Claudia M. Leite
MARTINEZ, Roberto
ROSSI, Marcos A.
SILVA, Joao Santana Da
Institución
Resumen
Paracoccidioidomycosis, the major systemic mycosis in Latin America, is caused by the thermally dimorphic fungus Paracoccidioides brasiliensis. To investigate the role of interleukin (IL)-12 in this disease, IL-12p40(-/-) deficient mice (IL-12p40(-/-)) and wild type mice (WT) were infected intravenously with viable yeast cells of P. brasiliensis 18 isolate. We found that, unlike WT mice, IL-12p40(-/-) mice did not control fungal proliferation and dissemination and succumbed to infection by day 21 after inoculation. Additionally, IL-12p40(-/-) mice presented a higher number of granulomas/mm(2) in lung tissue than WT mice, and showed unorganized granulomas containing high numbers of yeast cells. Moreover, IL-12p40(-/-) mice did not release detectable levels of IFN-gamma, but they produced high levels of IL-10, as well as IgG1 antibody. Additionally, splenocytes from both infected IL-12p40(-/-) and WT mice exhibited a suppressed Con-A-induced T cell proliferative response. Our findings suggest that the IL-12p40 subunit mediates resistance in paracoccidioidomycosis by inducting IFN-gamma production and a Th1 immune response