Artículos de revistas
Xenooplasmic Transfer between Buffalo and Bovine Enables Development of Homoplasmic Offspring
Fecha
2010Registro en:
CELLULAR REPROGRAMMING, v.12, n.3, p.231-236, 2010
2152-4971
10.1089/cell.2009.0076
Autor
CHIARATTI, Marcos Roberto
FERREIRA, Christina Ramires
MEIRELLES, Flavio Vieira
MEO, Simone Cristina
PERECIN, Felipe
SMITH, Lawrence Charles
FERRAZ, Marcio Leao
SA FILHO, Manoel Francisco de
GIMENES, Lindsay Unno
BARUSELLI, Pietro Sampaio
GASPARRINI, Bianca
GARCIA, Joaquim Mansano
Institución
Resumen
Nuclear-mitochondrial incompatibilities may be responsible for the development failure reported in embryos and fetuses produced by interspecies somatic cell nuclear transfer (iSCNT). Herein we performed xenooplasmic transfer (XOT) by introducing 10 to 15% of buffalo ooplasm into bovine zygotes to assess its effect on the persistence of buffalo mitochondrial DNA (mtDNA). Blastocyst rates were not compromised by XOT in comparison to both in vitro fertilized embryos and embryos produced by transfer of bovine ooplasm into bovine zygotes. Moreover, offspring were born after transfer of XOT embryos to recipient cows. Buffalo mtDNA introduced in zygotes was still present at the blastocyst stage (8.3 vs. 9.3%, p = 0.11), indicating unaltered heteroplasmy during early development. Nonetheless, no vestige of buffalo mtDNA was found in offspring, indicating a drift to homoplasmy during later stages of development. In conclusion, we show that the buffalo mtDNA introduced by XOT into a bovine zygote do not compromise embryo development. On the other hand, buffalo mtDNA was not inherited by offspring indicating a possible failure in the process of interspecies mtDNA replication.