Artículos de revistas
Cytotoxic effects of butanolic extract from Pfaffia paniculata (Brazilian Ginseng) on cultured human breast cancer cell line MCF-7
Fecha
2009Registro en:
EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY, v.61, n.1, p.75-82, 2009
0940-2993
10.1016/j.etp.2008.01.017
Autor
NAGAMINE, Marcia Kazumi
SILVA, Tereza Cristina da
MATSUZAKI, Patricia
PINELLO, Katia Cristina
COGLIATI, Bruno
PIZZO, Celia Regina
AKISUE, Gokithi
HARAGUCHI, Mitsue
GORNIAK, Silvana Lima
SINHORINI, Idercio Luiz
RAO, Kurapati Venkata Kesava
BARBUTO, Jose Alexandre Marzagao
DAGLI, Maria Lucia Zaidan
Institución
Resumen
Roots of Pfaffia paniculata have been well documented for multifarious therapeutic values and have also been used for cancer therapy in folk medicine. This study has been performed in a human breast tumor cell line, the MCF-7 cells. These are the most commonly used model of estrogen-positive breast cancer, and it has been originally established in 1973 at the Michigan Cancer Foundation from a pleural effusion taken from a woman with metastatic breast cancer. Butanolic extract of the roots of P. paniculata showed cytotoxic effect MCF-7 cell line. as determined with crystal violet assay, cellular death with acridine orange/ethidium bromide staining, and cell proliferation with immunocytochemistry of bromodeoxyuridine (BrdU). Subcellular alterations were evaluated by electron microscopy. Cells treated With butanolic extract showed degeneration of cytoplasmic components and profound morphological and nuclear alterations. The results show that this butanolic extract indeed presents cytotoxic substances, and its fractions merit further investigations. (C) 2008 Elsevier GmbH. All rights reserved.