Artículos de revistas
Genotyping of Brazilian Erysipelothrix spp. strains by amplified fragment length polymorphism
Fecha
2011Registro en:
JOURNAL OF MICROBIOLOGICAL METHODS, v.84, n.1, p.27-32, 2011
0167-7012
10.1016/j.mimet.2010.10.003
Autor
COUTINHO, Tania Alen
IMADA, Yumiko
BARCELLOS, David Emilio Santos Neves de
OLIVEIRA, Sergio Jose de
MORENO, Andrea Micke
Institución
Resumen
One hundred and fifty-one Erysipelothrix spp. isolates from diseased and carrier swine from Brazil were identified by PCR, submitted to serotyping and analyzed by amplified fragment length polymorphism with a single enzyme (AFLP). Reference strains from Australia and the United Kingdom were also examined. The 151 strains were classified into 18 different serotypes (1a, 1b, 2a, 2b, 4, 5, 6, 7, 8, 10, 11, 12, 15, 17, 19, 21, 24 and 25), being serotype 2b the most frequent (39.7%). By associating serotyping and PCR results, it was possible to identify 146 strains as E. rhusiopathiae and five strains as E. tonsillarum. Despite the fact that for this genus AFLP did not cluster all isolates according to serotype, origin, disease or isolation data, the execution of the technique was easy and fast, demonstrating high discriminatory power. The results produced by the AFLP analysis of Erysipelothrix spp. could also support its use as a discriminatory tool for E. rhusiopathiae and E. tonsillarum species. (C) 2010 Elsevier B.V. All rights reserved.