Artículos de revistas
Effects of the utilization of homeopathic elements in commercial diluent on swine sperm viability
Fecha
2011Registro en:
In Vitro Cellular & Developmental Biology - Animal, v.47, n.3, p.205-209, 2011
1071-2690
10.1007/s11626-011-9386-7
Autor
SOTO, Francisco Rafael Martins
VUADEN, Erlete Rosalina
COELHO, Cideli de Paula
BONAMIN, Leoni Villano
AZEVEDO, Sergio Santos de
BENITES, Nilson Roberti
BARROS, Flavia Regina Oliveira de
GOISSIS, Marcelo Demarchi
ASSUMPCAO, Mayra Elena Ortiz D`Avila
VISINTIN, Jose Antonio
MARQUES, Mariana Groke
Institución
Resumen
It has been speculated that the homeopathic treatment of sperm cells in order to improve semen quality could be promising. However, few data is available and its use in spermatozoa requires investigation. It is well established that mitochondrial membrane potential is an important viability parameter of spermatozoa and it is intimately related to reproductive efficiency. In this manner, new technologies in order to improve the activity of sperm cells and, finally, the fecundity of swine herds are of extremely importance. Due to the lack of knowledge of homeopathic treatment effect on spermatozoa, the aim of the present study was to verify the effect of three different homeopathic treatments on viability of boar sperm cells. Three homeopathic treatments composed by Pulsatila CH6, Pulsatila and Avena CH6, Avena CH6 and one control treatment (sucrose) were added to diluted boar semen, which were cooled for 24 or 48 h. Interestingly, no positive effect of homeopathic treatments was observed over semen viability. However, it was demonstrated that the 24 h of cooling storage provided more viable sperm cells when compared to the 48-h period. This effect of storage period on sperm viability was assessed by intact plasmatic membrane, intact acrosome and mitochondrial membrane potential evaluation.