Artículos de revistas
Integration pattern of HIV-1 based lentiviral vector carrying recombinant coagulation factor VIII in Sk-Hep and 293T cells
Fecha
2011Registro en:
BIOTECHNOLOGY LETTERS, v.33, n.1, p.23-31, 2011
0141-5492
10.1007/s10529-010-0387-5
Autor
RUSSO-CARBOLANTE, Elisa Maria de Sousa
PICANCO-CASTRO, Virginia
ALVES, Daiani Cristina Cilao
FERNANDES, Andrielle Castilho
ALMEIDA-PORADA, Graca
TONN, Torsten
COVAS, Dimas Tadeu
Institución
Resumen
293T and Sk-Hep-1 cells were transduced with a replication-defective self-inactivating HIV-1 derived vector carrying FVIII cDNA. The genomic DNA was sequenced to reveal LTR/human genome junctions and integration sites. One hundred and thirty-two sequences matched human sequences, with an identity of at least 98%. The integration sites in 293T-FVIIIDB and in Sk-Hep-FVIIIDB cells were preferentially located in gene regions. The integrations in both cell lines were distant from the CpG islands and from the transcription start sites. A comparison between the two cell lines showed that the lentiviral-transduced DNA had the same preferred regions in the two different cell lines.