Artículos de revistas
Poly(ethylene glycol) decorated poly(methylmethacrylate) nanoparticles for protein adsorption
Fecha
2011Registro en:
Materials science and engineering. C, Biomimetic materials, sensors and systems, v.31, n.3, p.562-566, 2011
0928-4931
10.1016/j.msec.2010.11.020
Autor
BONFA, Alfredo
SAITO, Rafael S. N.
FRANCA, Rafael F. O.
FONSECA, Benedito A. L.
PETRI, Denise F. S.
Institución
Resumen
Poly(ethylene glycol) decorated poly( methyl methacrylate) particles were synthesized by means of emulsion polymerization using poly(ethylene glycol) sorbitan monolaurate (Tween-20) as surfactant. PMMA/PEG particles presented mean diameter (195 +/- 15) nm, indicating narrow size distribution. The adsorption behavior of bovine serum albumin (BSA) and concanavalin A (ConA) onto PMMA/PEG particles was investigated by means of spectrophotometry. Adsorption isotherms obtained for BSA onto PMMA/PEG particles fitted well sigmoidal function, which is typical for multilayer adsorption. Con A adsorbed irreversibly onto PMMA/PEG particles. The efficiency of ConA covered particles to induce dengue virus quick agglutination was evaluated. (C) 2010 Elsevier B.V. All rights reserved.