Artículos de revistas
Apoptosis induction by (+)alpha-tocopheryl succinate in the absence or presence of all-trans retinoic acid and arsenic trioxide in NB4, NB4-R2 and primary APL cells
Fecha
2009Registro en:
LEUKEMIA RESEARCH, v.33, n.7, p.958-963, 2009
0145-2126
10.1016/j.leukres.2008.09.035
Autor
FREITAS, Rosana Aparecida
SANTOS, Guilherme Augusto Silva dos
TEIXEIRA, Hamilton Luiz Gimenes
SCHEUCHER, Priscila Santos
LUCENA-ARAUJO, Antonio Roberto
LIMA, Ana Silvia Gouveia
LIMA, Rodrigo Siqueira Abreu e
GARCIA, Aglair Bergamo
JORDAO JR., Alceu Afonso
FAICAO, Roberto Passetto
VANNUCCHI, Helio
REGO, Eduardo Magalhaes
Institución
Resumen
We analyzed the effect of (+)alpha-tocopheryl succinate (alpha-TOS) alone or associated with arsenic trioxide (ATO) or all-trans retinoid acid (ATRA) in acute promyelocytic leukemia (APL). alpha-TOS-induced apoptosis in APL clinical samples and in ATRA-sensitive (NB4) and ATRA-resistant (NB4-R2) APL cell lines. The effective dose 50% (ED-50) was calculated to be 71 and 58 mu M, for NB4 and NB4-R2, respectively. a-TOS neither induced nor modified ATRA-induced differentiation of APL cells, and did not affect the proliferation and differentiation of normal CD34(+) hematopoietic progenitors in methylcellulose assays. alpha-TOS exerted a moderate antagonistic effect to ATO-induced apoptosis when treatment was done simultaneously but when alpha-TOS was added 24 h after ATO, an additive effect was observed. Our results support the concept of alpha-TOS as an anti-leukemic compound which spares normal hematopoiesis. (C) 2008 Elsevier Ltd. All rights reserved.