Artículos de revistas
Oropouche virus entry into HeLa cells involves clathrin and requires endosomal acidification
Fecha
2008Registro en:
VIRUS RESEARCH, v.138, n.1/Fev, p.139-143, 2008
0168-1702
10.1016/j.virusres.2008.08.016
Autor
SANTOS, Rodrigo I. M.
RODRIGUES, Alcir H.
SILVA, Maria Lucia
MORTARA, Renato A.
ROSSI, Marcos A.
JAMUR, Maria Celia
OLIVER, Constance
ARRUDA, Eurico
Institución
Resumen
Oropouche virus (ORO), family Bunyaviridae, is the second most frequent cause of arboviral febrile illness in Brazil. Studies were conducted to understand ORO entry in HeLa cells. Chlorpromazine inhibited early steps of ORO replication cycle, consistent with entry/uncoating. The data indicate that ORO enters HeLa cells by clathrin-coated vesicles, by a mechanism susceptible to endosomal acidification inhibitors. Transmission electron microscopy and immunofluorescence indicated that ORO associates with clathrin-coated pits and can be found in association with late endosomes in a time shorter than 1 h. (C) 2008 Elsevier B.V. All rights reserved.