Artículos de revistas
Evaluation of rhBMP-2 and Natural Latex as Potential Osteogenic Proteins in Critical Size Defects by Histomorphometric Methods
Fecha
2010Registro en:
The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology, v.293, n.5, p.794-801, 2010
1932-8486
10.1002/ar.21097
Autor
ISSA, Joao Paulo Mardegan
DEFINO, Helton Luiz Aparecido
NETTO, Joaquim Coutinho
VOLPON, Jose Batista
REGALO, Simone Cecilio Hallak
IYOMASA, Mamie Mizusaki
SIESSERE, Selma
TIOSSI, Rodrigo
Institución
Resumen
This in vivo study evaluated the osteogenic potential of two proteins, recombinant human bone morphogenetic protein-2 (rhBMP-2) and a protein extracted from natural latex (Hevea brasiliensis, P-1), and compared their effects on bone defects when combined with a carrier or a collagen gelatin. Eighty-four (84) Wistar rats were divided into two groups, with and without the use of collagen gelatin, and each of these were divided into six treatment groups of seven animals each. The treatment groups were: (1) 5 mu g of pure rhBMP-2; (2) 5 mu g of rhBMP-2/monoolein gel; (3) pure monoolein gel; (4) 5 mu g of pure P-1; (5) 5 mu g of P-1/monoolein gel; (6) critical bone defect control. The animals were anesthetized and a 6 mm diameter critical bone defect was made in the left posterior region of the parietal bone. Animals were submitted to intracardiac perfusion after 4 weeks and the calvaria tissue was removed for histomorphometric analysis. In this experimental study, it was concluded that rhBMP-2 allowed greater new bone formation than P-1 protein and this process was more effective when the bone defect was covered with collagen gelatin (P < 0.05). Anat Rec, 293:794-801, 2010. (C) 2010 Wiley-Liss, Inc.