Artículos de revistas
Stereoselective analysis of carvedilol in human plasma and urine using HPLC after chiral derivatization
Fecha
2008Registro en:
BIOPHARMACEUTICS & DRUG DISPOSITION, v.29, n.5, p.280-288, 2008
0142-2782
10.1002/bdd.613
Autor
PECCININI, Rosangela G.
XIMENES, Valdecir F.
CESARINO, Evandro J.
LANCHOTE, Vera L.
Institución
Resumen
An enantioselective high-performance liquid chromatographic method for the analysis of carvedilol in plasma and urine was developed and validated using (-)-menthyl chloroformate (MCF) as a derivatizing reagent. Chloroform was used for extraction, and analysis was performed by HPLC on a C18 column with a fluorescence detector. The quantitation limit was 0.25 ng/ml for S(-)-carvedilol in plasma and 0.5 ng/ml for R(+)-carvedilol in plasma and for both enantiomers in urine. The method was applied to the study of enantioselectivity in the pharmacokinetics of carvedilol administered in a multiple dose regimen (25mg/12h) to a hypertensive elderly female patient. The data obtained demonstrated highest plasma levels for the R(+)-carvedilol(AUCSS 75.64 vs 37.29ng/ml). The enantiomeric ratio R(+)/S(-) was 2.03 for plasma and 1.49 0 - 12 for urine (Aeo-12 17.4 vs 11.7 pg). Copyright (c) 2008 John Wiley & Sons, Ltd.