Artículos de revistas
Trypanosoma cruzi: evaluation of (-)-cubebin derivatives activity in the messenger RNAs processing
Fecha
2011Registro en:
PARASITOLOGY RESEARCH, v.109, n.2, p.445-451, 2011
0932-0113
10.1007/s00436-011-2275-x
Autor
SILVA, Marcio Luis Andrade e
CICARELLI, Regina Maria Barretto
PAULETTI, Patricia M.
LUZ, Priscilla Paiva
REZENDE, Karen Cristina Souza
JANUARIO, Ana Helena
SILVA, Rosangela da
PEREIRA, Ana Carolina
BASTOS, Jairo Kenupp
ALBUQUERQUE, Sergio de
MAGALHAES, Lizandra Guidi
CUNHA, Wilson Roberto
Institución
Resumen
No fully effective treatment has been developed since the discovery of Chagas` disease. Since drug-resistant Trypanosoma cruzi strains are occurring and the current therapy is effective in the acute phase but with various adverse side effects, more studies are needed to characterize the susceptibility of T. cruzi to new drugs. Pre-mRNA maturation in trypanosomatids occurs through a process called trans-splicing, which is unusual RNA processing reaction, and it implies the processing of polycistronic transcription units into individual mRNAs; a short transcript spliced leader (SL RNA) is trans-spliced to the acceptor pre-mRNA, giving origin to the mature mRNA. Cubebin derivatives seem to provide treatments with less collateral effects than benznidazole and showed similar or better trypanocidal activities than benznidazole. Therefore, the cubebin derivatives ((-)-6,6`-dinitrohinokinin (DNH) and (-)-hinokinin (HQ)) interference in the mRNA processing was evaluated using T. cruzi permeable cells (Y and BOL (Bolivia) strains) following by RNase protection reaction. These substances seem to intervene in any step of the RNA transcription, promoting alterations in the RNA synthesis, even though the RNA processing mechanism still occurs. Furthermore, HQ presented better activity against the parasites than DNH, meaning that BOL strain seems to be more resistant than Y.