Artículos de revistas
Cytotoxic activity of a dichloromethane extract and fractions obtained from Eudistoma vannamei (Tunicata: Ascidiacea)
Fecha
2008Registro en:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v.151, n.3, Special Issue, p.391-398, 2008
1095-6433
10.1016/j.cbpa.2007.02.018
Autor
JIMENEZ, Paula C.
WILKE, Diego V.
TAKEARA, Renata
LOTUFO, Tito M. C.
PESSOA, Claudia
MORAES, Manoel Odorico de
LOPES, Norberto P.
COSTA-LOTUFO, Leticia V.
Institución
Resumen
This study consists of the bioassay-guided fractionation of the dichloromethane extract from Eudistoma vannamei and the pharmacological characterization of the active fractions. The dried hydromethanolic extract dissolved in aqueous methanol was partitioned with dichloromethane and chromatographed on a silica gel flash column. The anti-proliferative effect was monitored by the MTT assay. Four of the latest fractions, numbered 14 to 17, which held many chemical similarities amongst each other, were found to be the most active. The selected fractions were tested for viability, proliferation and death induction on cultures of HL-60 promycloblastic leukemia cells. The results suggested that the observed cytotoxicity is related to apoptosis induction. (C) 2007 Elsevier Inc. All rights reserved.