Artículos de revistas
Enantioselective analysis of oxybutynin and N-desethyloxybutynin with application to an in vitro biotransformation study
Fecha
2008Registro en:
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v.875, n.1, p.161-167, 2008
1570-0232
10.1016/j.jchromb.2008.05.023
Autor
FONSECA, Patricia da
FREITAS, Luis Alexandre Pedro de
PINTO, Luis Felipe Ribeiro
PESTANA, Cezar Rangel
BONATO, Pierina Sueli
Institución
Resumen
An enantioselective method using liquid-phase microextraction (LPME) followed by HPLC analysis was developed for the determination of oxybutynin (OXY) and its major metabolite N-desethyloxybutynin (DEO) in rat liver microsomal fraction. The LPME procedure was optimized using multifactorial experiments. Under the optimal extraction conditions, the mean recoveries were 61 and 55% for (R)-OXY and (S)-OXY, respectively. and 70 and 76% for (R)-DEO and (S)-DEO, respectively. The validated method was employed to an in vitro biotransformation study using rat liver microsomal fraction. The results demonstrated the enantioselective biotransformation of OXY. (c) 2008 Elsevier B.V. All rights reserved.