Artículos de revistas
In vitro safety assessment of papain on human skin: A qualitative Light and Transmission Electron Microscopy (TEM) study
Fecha
2008Registro en:
Revista Brasileira de Ciências Farmacêuticas/Brazilian Journal of Pharmaceutical Sciences, v.44, n.1, p.151-156, 2008
1516-9332
Autor
LOPES, Patricia Santos
RUAS, Gabriele Wander
BABY, Andre Rolim
PINTO, Claudineia Aparecida Sales de Oliveira
WATANABE, Ii-sei
VELASCO, Maria Valeria Robles
KANEKO, Telma Mary
Institución
Resumen
Papain is a thiol proteolytic enzyme widely used in dermatology that found applications in wound treatment. Recently, papain was also used as absorption enhancer which can modify the peptide/ protein material in the bilayer domain. We investigated papain safety using human skin that was exposed to papain in vitro at different times: 4, 24 and 48 hours. The samples were examined using Light and Transmission Electron Microscopy (TEM) to study of the mechanisms involved in enhancer-skin interaction. After 24 hours, changes occurred in corneosomes. However, samples of 48 hours did not show major changes in agreement with the control. These findings indicated that papain could be used safely onto the skin.