Artículos de revistas
Synthetic peptides as an antigenic base in an ELISA for laboratory diagnosts of schistosomiasis mansoni
Fecha
2008Registro en:
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, v.102, n.4, p.360-366, 2008
0035-9203
10.1016/j.trstmh.2007.11.008
Autor
OLIVEIRA, Edward Jose de
KANAMURA, HerminiaYohko
Kioko Takei
HIRATA, Rosario Dominguez Crespo
VALLI, Luiz Carlos Pedrosa
NGUYEN, Nga Yen
RODRIGUES, Izabel de Carvalho
JESUS, Amelia Ribeiro de
HIRATA, Mario Hiroyuki
Institución
Resumen
A pool of five synthetic peptides was used as an antigenic base in an ELISA (ELISA-Pp) for laboratory diagnosis of Schistosoma mansoni. Serum samples were obtained from individuals with acute (n = 23) and chronic (n = 30) schistosomiasis, with other parasitoses (n = 39) or without parasitic infections (n = 100). ELISA-Pp was compared with other immunoenzymatic methods for detection of IgM (IgM-ELISA) or IgG (IgG-ELISA) as well as an immunofluorescence test for detection of IgM antibodies (IgM-IFT). The sensitivity and specificity of ELISA-Pp was 86.8% and 94.2% when tested on the schistosomiasis group and the non-schistosomiasis group, respectively. Comparison of ELISA-Pp with other serological methods resulted in K concordance indices varying from 0.59 to 0.75. Evaluation of anti-peptide IgG antibodies showed higher Levels in patients with acute compared with chronic schistosomiasis (P = 0.001). ELISA-Pp showed satisfactory sensitivity and high specificity and may constitute a potentially useful method for laboratory diagnosis of schistosomiasis mansoni. (c) 2007 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.