Artículos de revistas
Bacterial community in the rhizosphere and rhizoplane of wild type and transgenic eucalyptus
Fecha
2009Registro en:
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v.25, n.6, p.1065-1073, 2009
0959-3993
10.1007/s11274-009-9990-9
Autor
ANDREOTE, Fernando D.
ROSSETTO, Priscilla B.
MENDES, Rodrigo
AVILA, Luciana A.
LABATE, Carlos A.
PIZZIRANI-KLEINER, Aline A.
AZEVEDO, Joao L.
ARAUJO, Welington L.
Institución
Resumen
The rhizosphere is a niche exploited by a wide variety of bacteria. The expression of heterologous genes by plants might become a factor affecting the structure of bacterial communities in the rhizosphere. In a greenhouse experiment, the bacterial community associated to transgenic eucalyptus, carrying the Lhcb1-2 genes from pea (responsible for a higher photosynthetic capacity), was evaluated. The culturable bacterial community associated to transgenic and wild type plants were not different in density, and the Amplified Ribosomal DNA Restriction Analysis (ARDRA) typing of 124 strains revealed dominant ribotypes representing the bacterial orders Burkholderiales, Rhizobiales, and Actinomycetales, the families Xanthomonadaceae, and Bacillaceae, and the genus Mycobacterium. Principal Component Analysis based on the fingerprints obtained by culture-independent Denaturing Gradient Gel Electrophoresis analysis revealed that Alphaproteobacteria, Betaproteobacteria and Actinobacteria communities responded differently to plant genotypes. Similar effects for the cultivation of transgenic eucalyptus to those observed when two genotype-distinct wild type plants are compared.