Artículos de revistas
Enhancement of europium emission band of europium tetracycline complex in the presence of cholesterol
Fecha
2008Registro en:
JOURNAL OF FLUORESCENCE, v.18, n.1, p.169-174, 2008
1053-0509
10.1007/s10895-007-0260-7
Autor
SILVA, Flavia Rodrigues de Oliveira
SAMAD, Ricardo Elgul
GOMES, Laercio
COURROL, Lilia Coronato
Institución
Resumen
We report here the observation, for the first time, of the enhancement of Europium-Tetracycline complex emission in cholesterol solutions. This enhancement was initially observed with the addition of the enzyme cholesterol oxidase, which produces H2O2, the agent driver of the Europium tetracycline complex, to the solution. However, it was found that the enzyme is not needed to enhance the luminescence. A calibration curve was determined, resulting in a simple method to measure the cholesterol quantity in a solution. This method shows that the complex can be used as a sensor to determine cholesterol in biological systems.