Artículos de revistas
LOW-INTENSITY PULSED ULTRASOUND PRODUCED AN INCREASE OF OSTEOGENIC GENES EXPRESSION DURING THE PROCESS OF BONE HEALING IN RATS
Fecha
2010Registro en:
ULTRASOUND IN MEDICINE AND BIOLOGY, v.36, n.12, p.2057-2064, 2010
0301-5629
10.1016/j.ultrasmedbio.2010.07.012
Autor
FAVARO-PIPI, Elaine
BOSSINI, Paulo
OLIVEIRA, Poliani de
RIBEIRO, Juliana Uema
TIM, Carla
PARIZOTTO, Nivaldo A.
ALVES, Jose Marcos
RIBEIRO, Daniel Araki
ARAUJO, Heloisa Sobreiro Selistre de
RENNO, Ana Claudia Muniz
Institución
Resumen
The aim of this study was to measure the temporal expression of osteogenic genes during the process of bone healing in low-intensity pulsed ultrasound (LIPUS) treated bone defects by means of histopathologic and real-time polymerase chain reaction (PCR) analysis. Animals were randomly distributed into two groups (n = 30): control group (bone defect without treatment) and LIPUS treated (bone defect treated with LIPUS). On days 7, 13 and 25 postinjury, 10 rats per group were sacrificed. Rats were treated with a 30 mW/cm(2) LIPUS. The results pointed out intense new bone formation surrounded by highly vascularized connective tissue presenting a slight osteogenic activity, with primary bone deposition was observed in the group exposed to LIPUS in the intermediary (13 days) and late stages of repair (25 days) in the treated animals. In addition, quantitative real-time polymerase chain reaction (RT-qPCR) showed an upregulation of bone morphogenetic protein 4 (BMP4), osteocalcin and Runx2 genes 7 days after the surgery. In the intermediary period, there was no increase in the expression. The expression of alkaline phosphatase, BMP4 and Runx2 was significantly increased at the last period. Our results indicate that LIPUS therapy improves bone repair in rats and upregulated osteogenic genes, mainly at the late stages of recovery. (E-mail: a.renno@unifesp.br) (C) 2010 Published by Elsevier Inc. on behalf of World Federation for Ultrasound in Medicine & Biology.