Artículos de revistas
Single reaction interface flow system for chemiluminescent monitoring of mannitol based on its hydroxyl radical scavenger activity
Fecha
2008Registro en:
TALANTA, v.77, n.2, p.518-521, 2008
0039-9140
10.1016/j.talanta.2008.03.021
Autor
SILVESTRE, Cristina I. C.
SANTOS, Joao L. M.
LIMA, Jose L. F. C.
ZAGATTO, Elias A. G.
Institución
Resumen
A single reaction interface flow analysis (SIFA) system for the monitoring of mannitol in pharmaceutical formulations and human urine is presented. The developed approach takes advantage of the mannitol scavenger aptitude to inhibit the chemiluminescent reaction between luminol and myoglobin in the absence of H(2)O(2). The SIFA system facilitated the fully automation of the developed methodology, allowing the in-line reproducible handling of chemical species with a very short lifetime as is the case of the hydroxyl radical generated in the abovementioned luminol/myoglobin reaction. The proposed methodology allowed the determination of mannitol concentrations between 25 mmol L(-1) and 1 mol L(-1), with good precision (R.S.D. < 4.7%, n = 3) and a sampling frequency of about 60 h(-1). The procedure was applied to the determination of mannitol in pharmaceuticals and in human urine samples Without any pretreatment process. The results obtained for pharmaceutical formulations were statistically comparable to those provided by the reference method (R.D. < 4.6%); recoveries values obtained in the analysis of spiked urine samples (between 94.9 and 105.3% of the added amount) were also satisfactory. (C) 2008 Elsevier B.V. All rights reserved.