Artículos de revistas
In vitro organogenesis of Passiflora alata
Fecha
2010Registro en:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, v.46, n.1, p.28-33, 2010
1054-5476
10.1007/s11627-009-9251-5
Autor
PINTO, Ana Paula Chiaverini
MONTEIRO-HARA, Alessandra Cristina B. A.
STIPP, Liliane Cristina Liborio
MENDES, Beatriz Madalena Januzzi
Institución
Resumen
Passiflora alata in vitro organogenesis was studied based on explant type, culture medium composition, and incubation conditions. The results indicated that the morphogenic process occurred more efficiently when hypocotyl segment-derived explants were cultured in media supplemented with cytokinin and AgNO(3) incubated under a 16-h photoperiod. The shoot bud elongation and plant development were obtained by transferring the material to MSM culture medium supplemented with GA(3) and incubated in flasks with vented lids. Histological analyses of the process revealed that the difficulties in obtaining plants could be related to the development of protuberances and leaf primordia structures, which did not contain shoot apical meristem. Roots developed easily by transferring elongated shoots to 1/2 MSM culture medium. Plant acclimatization occurred successfully, and somaclonal variation was not visually detected. The efficiency of this organogenesis protocol will be evaluated for genetic transformation of this species to obtain transgenic plants expressing genes that can influence the resistance to Cowpea aphid borne mosaic virus.