Artículos de revistas
Effects of Aflatoxin B(1) and Fumonisin B(1) on the Viability and Induction of Apoptosis in Rat Primary Hepatocytes
Fecha
2010Registro en:
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.11, n.4, p.1944-1955, 2010
1422-0067
10.3390/ijms11041944
Autor
RIBEIRO, Deise H. B.
FERREIRA, Fabiane L.
SILVA, Valeria N. da
AQUINO, Simone
CORREA, Benedito
Institución
Resumen
The present study evaluated the effect of aflatoxin B(1) (AFB(1)) and fumonisin B(1) (FB(1)) either alone, or in association, on rat primary hepatocyte cultures. Cell viability was assessed by flow cytometry after propidium iodine intercalation. DNA fragmentation and apoptosis were assessed by agarose gel electrophoresis and acridine orange and ethidium bromide staining. At the concentrations of AFB(1) and FB(1) used, the toxins did not decrease cell viability, but did induce apoptosis in a concentration and time-dependent manner.