Artículos de revistas
Correlation of meta 1 expression with culture stage, cell morphology and infectivity in Leishmania (Leishmania) amazonensis promastigotes
Fecha
2011Registro en:
Memórias do Instituto Oswaldo Cruz, v.106, n.2, p.190-193, 2011
0074-0276
10.1590/S0074-02762011000200012
Autor
SANTOS, Marcos Gonzaga dos
SILVA, Maria Fernanda Laranjeira da
ZAMPIERI, Ricardo Andrade
LAFRAIA, Rafaella Marino
FLOETER-WINTER, Lucile Maria
Institución
Resumen
The parasitic protozoan Leishmania (Leishmania) amazonensis alternates between mammalian and insect hosts. In the insect host, the parasites proliferate as procyclic promastigotes andthen differentiate into metacyclic infective forms. The meta 1 gene is preferentially expressed during metacyclogenesis. Meta 1 expression profile determination along parasite growth curves revealed that the meta 1 mRNA level peaked at the early stationary phase then decreased to an intermediate level. No correlation was observed between meta 1 expression and infectivity. Conversely, infectivity correlated with the increase of apoptotic cells in the late stationary phase.