DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients

dc.creatorLIMA, Patrícia Santos Pereira
dc.creatorMOLFFETA, Greice Andreoti
dc.creatorARAUJO, Amélia Góes de
dc.creatorZAGO, Marco Antônio
dc.creatorSILVA JR., Wilson Araújo da
dc.date.accessioned2012-03-26T16:58:11Z
dc.date.accessioned2018-07-04T14:06:28Z
dc.date.available2012-03-26T16:58:11Z
dc.date.available2018-07-04T14:06:28Z
dc.date.created2012-03-26T16:58:11Z
dc.date.issued2008
dc.identifierGenetics and Molecular Biology, v.31, n.3, p.632-638, 2008
dc.identifier1415-4757
dc.identifierhttp://producao.usp.br/handle/BDPI/7601
dc.identifier10.1590/S1415-47572008000400005
dc.identifierhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400005
dc.identifierhttp://www.scielo.br/pdf/gmb/v31n3/a05v31n3.pdf
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1605971
dc.description.abstractThe aim of this work was to evaluate the methylation profile of the p15 (CDKN2B) gene in Brazilian patients with leukemia and to correlate the CDKN2B gene expression with the percentage of methylated CpG dinucleotides in its promoter region. Thirty-one samples from six patients with acute lymphocytic leukemia (ALL), four with chronic myeloid leukemia (CML), and 21 with acute myeloid leukemia (AML) were evaluated by MSP (Methylation-Specific PCR). The CDKN2B gene was found to be methylated in four (67%) of the six ALL samples and in 16 (76%) of the 21 AML samples, but in none of the four CML samples analyzed. We observed a correlation between the CDKN2B mRNA expression (RT-PCR) and the percentage of methylated CpG dinucleotides. Therefore, this study in Brazilian patients confirms that the CDKN2B gene is methylated in the majority of leukemia patients.
dc.languageeng
dc.publisherSociedade Brasileira de Genética
dc.relationGenetics and Molecular Biology
dc.rightsCopyright Sociedade Brasileira de Genética
dc.rightsopenAccess
dc.subjectCpG island
dc.subjectDNA methylation
dc.subjectCDKN2B
dc.titleDNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución