Artículos de revistas
Hypothalamic Stearoyl-coa Desaturase-2 (scd2) Controls Whole-body Energy Expenditure
Registro en:
International Journal Of Obesity. Nature Publishing Group, v. 40, p. 471 - 478, 2016.
0307-0565
1476-5497
WOS:000371799300011
10.1038/ijo.2015.188
Autor
de Moura
R. F.; Nascimento
L. F.; Ignacio-Souza
L. M.; Morari
J.; Razolli
D. S.; Solon
C.; de Souza
G. F. P.; Festuccia
W. T.; Velloso
L. A.
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Stearoyl-CoA desaturase-2 (SCD2) is the main delta 9 desaturase expressed in the central nervous system. Because of its potential involvement in controlling whole-body adiposity, we evaluated the expression and function of SCD2 in the hypothalami of mice. SUBJECTS/METHODS: Male mice of different strains were used in real-time PCR, immunoblot and metabolic experiments. In addition, antisense oligonucleotides and lentiviral vectors were used to reduce and increase the expression of SCD2 in the hypothalamus. RESULTS: The level of SCD2 in the hypothalamus is similar to other regions of the central nervous system and is similar to 10-fold higher than in any other region of the body. In the arcuate nucleus, SCD2 is expressed in proopiomelanocortin and neuropeptide-Y neurons. Upon high fat feeding, the level of hypothalamic SCD2 increases. Inhibition of hypothalamic SCD2 as accomplished by two distinct approaches, an antisense oligonucleotide or a short-hairpin RNA delivered by a lentivirus, resulted in reduced body mass gain mostly due to increased energy expenditure and increased spontaneous activity. Increasing hypothalamic SCD2 by a lentivirus approach resulted in no change in body mass and food intake. CONCLUSIONS: Thus, SCD2 is highly expressed in the hypothalami of rodents and its knockdown reduces body mass due to increased whole-body energy expenditure. 40 3 471 478 Sao Paulo Research Foundation Conselho Nacional de Desenvolvimento Cientifico e Tecnologico Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)