Artículos de revistas
Microfluidic Tools Toward Industrial Biotechnology
Registro en:
Biotechnology Progress. Wiley-blackwell, v. 32, p. 1372 - 1389, 2016.
8756-7938
1520-6033
WOS:000392816300002
10.1002/btpr.2350
Autor
Oliveira
Aline F.; Pessoa
Amanda C. S. N.; Bastos
Reinaldo G.; de la Torre
Lucimara G.
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Microfluidics is a technology that operates with small amounts of fluids and makes possible the investigation of cells, enzymes, and biomolecules and encapsulation of biocatalysts in a greater variety of conditions than permitted using conventional methods. This review discusses technological possibilities that can be applied in the field of industrial biotechnology, presenting the principal definitions and fundamental aspects of microfluidic parameters to better understand advanced approaches. Specifically, concentration gradient generators, droplet-based microfluidics, and microbioreactors are explored as useful tools that can contribute to industrial biotechnology. These tools present potential applications, inclusive as commercial platforms to optimizing in bioprocesses development as screening cells, encapsulating biocatalysts, and determining critical kinetic parameters. (C) 2016 American Institute of Chemical Engineers 32 6 1372 1389 Sao Paulo Research Foundation (FAPESP) [2014/26814-7] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)